Peptide Dosage Reference & Reconstitution Guide
Calculate reconstitution concentrations and find research dosage references.
Reconstitution Calculator
Lyophilized (freeze-dried) peptides must be mixed with bacteriostatic water before use. The amount of water you add determines the concentration.
Concentration: 25 mcg per unit
Units to draw: 10 units
Volume: 0.10 mL
The Formula: Concentration (mcg/unit) = Peptide Amount (mcg) / Water Added (units)
100 units = 1 mL on a standard insulin syringe
100 units = 1 mL on a standard insulin syringe
Common Reconstitution Examples
BPC-157 (5mg vial)
| Water Added | Concentration | 250 mcg dose | 500 mcg dose |
|---|---|---|---|
| 1 mL | 50 mcg/unit | 5 units | 10 units |
| 2 mL | 25 mcg/unit | 10 units | 20 units |
| 3 mL | 16.7 mcg/unit | 15 units | 30 units |
TB-500 (5mg vial)
| Water Added | Concentration | 250 mcg dose | 500 mcg dose |
|---|---|---|---|
| 1 mL | 50 mcg/unit | 50 units (2.5mg) | 100 units (full syringe) |
| 2 mL | 25 mcg/unit | 100 units (2.5mg) | Use 2 syringes |
Ipamorelin (5mg vial)
| Water Added | Concentration | 250 mcg dose | 500 mcg dose |
|---|---|---|---|
| 2 mL | 25 mcg/unit | 8 units (200mcg) | 12 units (300mcg) |
| 2.5 mL | 20 mcg/unit | 10 units (200mcg) | 15 units (300mcg) |
Semaglutide (5mg vial)
| Water Added | Concentration | 250 mcg dose | 500 mcg dose |
|---|---|---|---|
| 2 mL | 25 mcg/unit | 10 units (0.25mg) | 20 units (0.5mg) |
| 2.5 mL | 20 mcg/unit | 12.5 units (0.25mg) | 25 units (0.5mg) |
Research Dosage Reference
Critical Disclaimer: The dosages listed below are compiled from published preclinical research, anecdotal community reports, and (where applicable) approved pharmaceutical labeling. They are NOT medical recommendations. PeptideExaminer does not recommend self-administration of any research peptide. These figures are provided as educational reference only.
Recovery & Healing Peptides
| Peptide | Common Dose | Frequency | Route | Notes |
|---|---|---|---|---|
| BPC-157 | 200-500 mcg | 1-2x daily | SubQ or oral | Lower end for maintenance; higher for acute injury protocols |
| TB-500 | 2-2.5 mg | 2x/week (loading); 1x/week (maintenance) | SubQ | Loading phase typically 4-6 weeks |
| GHK-Cu | 1-2 mg | 1x daily | SubQ | Also available topically (1-3% cream) |
| KPV | 200-500 mcg | 1-2x daily | SubQ or oral | Oral may be effective for gut-specific applications |
Growth Hormone Secretagogues
| Peptide | Common Dose | Frequency | Route | Notes |
|---|---|---|---|---|
| Ipamorelin | 200-300 mcg | 2-3x daily | SubQ | Best before bed and/or post-workout |
| CJC-1295 (no DAC) | 100-200 mcg | 2-3x daily | SubQ | Typically paired with ipamorelin |
| CJC-1295 (with DAC) | 1-2 mg | 1-2x/week | SubQ | Long half-life; do not combine with no-DAC version |
Anti-Aging
| Peptide | Common Dose | Frequency | Route | Notes |
|---|---|---|---|---|
| Epithalon | 5-10 mg | Daily for 10-20 day cycles | SubQ | Typically cycled: 10-20 days on, 4-6 months off |
Sexual Health
| Peptide | Common Dose | Frequency | Route | Notes |
|---|---|---|---|---|
| PT-141 (Bremelanotide) | 1.75 mg (FDA-approved) | On-demand, max 1x/day | SubQ | Max 8 doses/month per FDA label; nausea is common |
Metabolic / Weight Loss
| Peptide | Common Dose | Frequency | Route | Notes |
|---|---|---|---|---|
| Semaglutide (Wegovy) | 0.25 mg starting | 1x/week | SubQ | Titrate up to 2.4 mg over weeks |
| Tirzepatide (Zepbound) | 2.5 mg starting | 1x/week | SubQ | Titrate up to 15 mg over weeks |
Syringe Reading Guide
100-Unit (1 mL)
- Each small line = 1 unit (0.01 mL)
- Each numbered line = 10 units (0.1 mL)
- Full syringe = 100 units (1 mL)
Standard size, good for most doses
50-Unit (0.5 mL)
- Each small line = 1 unit (0.01 mL)
- Each numbered line = 5 units (0.05 mL)
- Full syringe = 50 units (0.5 mL)
Better accuracy for small doses
30-Unit (0.3 mL)
- Each small line = 0.5 units (0.005 mL)
- More precise markings
- Full syringe = 30 units (0.3 mL)
Most precise for very small doses
Tip: For doses under 10 units, use a 30-unit syringe for better accuracy. For doses over 50 units, use a 100-unit syringe.
Reconstitution Best Practices
What You Need
- Lyophilized peptide vial
- Bacteriostatic water (BAC water) — NOT sterile water, NOT saline
- Insulin syringes (100-unit, 1 mL)
- Alcohol swabs
- Clean workspace
Step-by-Step
- Clean: Wipe the tops of both the peptide vial and BAC water vial with alcohol swabs
- Draw water: Using an insulin syringe, draw your chosen amount of BAC water
- Add slowly: Insert the needle into the peptide vial and let the water run down the SIDE of the vial — do NOT squirt directly onto the powder
- Don't shake: Gently roll the vial between your palms or let it sit in the refrigerator for 15-30 minutes. Shaking can denature the peptide.
- Check clarity: The solution should be clear and colorless. Cloudiness or particles indicate contamination or degradation — discard.
- Store: Refrigerate at 2-8 C. Use within 2-4 weeks.
Common Mistakes
- Using too little water: Creates concentrated solutions where small syringe errors = large dose errors
- Using too much water: Creates dilute solutions requiring large injection volumes
- Squirting water onto powder: Can damage peptide through mechanical stress
- Shaking vigorously: Denatures peptides
- Using sterile water instead of BAC water: No preservative = bacterial growth risk
- Not swabbing vial tops: Introduces contaminants
- Reusing needles: Dulls the needle and introduces bacteria